Combined effects of the pesticide spinetoram and the cyanobacterium Microcystis on the water flea Daphnia pulex.

Spinetoram is one of the most worldwidely used pesticides for its high insecticidal efficacy and low human toxicity. Following the large usage of spinetoram, the ecotoxicity and environmental risks to aquatic ecosystems have call for urgent study. In the present study, we investigated the combined effects of spinetoram and the harmful alga Microcystis aeruginosa in freshwater, on survival and reproduction of Daphnia pulex. Acute toxicity test of spinetoram resulted in negative effects on survival, with a 48-h LC50 value of 37.71 µg L-1. Under the long-time exposure to environmentally relevant concentrations (0.18 and 0.35 µg L-1) of spinetoram and a low composition of Microcystis (30%) in the diet, D. pulex showed both shorter longevity and lower fecundity; the time to first brood also increased. At population level, carrying capacity was highly decreased by spinetoram and Microcystis, whereas a significant decrease of intrinsic growth rate was observed at 0.35 µg L-1 spinetoram with 30% Microcystis as food. The present study highlighted that pesticide spinetoram had highly toxic effects on D. pulex and could reduce the tolerance of D. pulex to M. aeruginosa, causing great effects on D. pulex population in natural waterbodies.

Stress-responsive expression of a glutathione S-transferase (delta) gene in waterflea Daphnia magna challenged by microcystin-producing and microcystin-free Microcystis aeruginosa.

Harmful cyanobacterial blooms resulting from eutrophication and global warming have emerged as a worldwide environmental concern. Some zooplankton populations, including Daphnia, have been shown to adapt locally to microcystin-producing Microcystis. Previous in vitro experiments indicate that glutathione-S-transferase (GST) may act as the first step of detoxification in Daphnia by conjugating microcystins (MCs) with glutathione. The GST family is categorized into many classes, and different classes present distinct responses to MC detoxification. To date, however, the molecular mechanism of single class GST participation in buffering the toxic effects of MCs in Daphnia remains poorly known. In this study, a full-length delta-GST cDNA of Daphnia magna (Dm-dGST) was isolated and characterized through bioinformatics. Differential gene expression studies revealed that short-term exposure to microcystin-producing (MP) Microcystis aeruginosa increased Dm-dGST transcript levels. By contrast, long-term exposure to MP or microcystin-free (MF) M. aeruginosa decreased Dm-dGST transcript levels. Together with changes in three other antioxidation biomarkers (catalase, CuZn- and Mn-superoxide dismutase), it is concluded that Dm-dGST can potentially biotransform MCs to reduce their toxicity. The present study highlights the importance of Dm-dGST in response to MC toxicity and may thus facilitate future research on the molecular mechanisms of MC tolerance in zooplankton under an increasing eutrophic world.